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Preheat oven to 375°F. Prepare 24 cavity muffin pan with vegetable pan spray.
Brush ham slices evenly with a thin layer of mustard. Place 1 slice in each of the prepared pan cavities to form a cup, mustard side facing up. Crack an egg into each of the cups and sprinkle evenly with salt and pepper.
Bake 14-16 minutes or until eggs have reached desired degree of doneness, rotating pan halfway through baking. Carefully remove from pan and sprinkle evenly with chopped chives. Serve warm.